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| Menu, Bruno; Peruzzi, Stefano; Vergnet, Alain; Vidal, Marie-odile; Chatain, Beatrice. |
| The objective of this study was to develop a rapid and effective method of sexing juvenile sea bass with minimum labour and material. To this end, the gonad squash mount technique was applied along with macroscopic techniques for sexing a large number of experimental fish at the age of 215-275 days post fertilization (p.f.). At this age, 90% of the 3894 fish could be unambiguously sexed by macroscopic examination of their gonads, whereas the remaining proportion was identified using squash mount preparations (10%). The accuracy of the observations was measured up to classical histology procedures. Undifferentiated fish accounted for 0.1% of the total population only. The application of the squash mount technique holds out the prospect of considerable... |
| Tipo: Text |
Palavras-chave: Histology; Squash; Juvenile; Gonads; Seabass; Dicentrarchus labrax. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-378.pdf |
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| Fauvel, Christian; Suquet, Marc; Dreanno, Catherine; Zonno, Vincenzo; Menu, Bruno. |
| A sperm cryopreservation protocol adapted from turbot, was tested on sea bass using either 250-mu L straws or 1.5-mL cryovials. A dilution to 1/3 in Mounib's extender and a cooling rate of 65 degrees C.min(-1) allowed frozen sperm to recover an initial motility similar to that of fresh sperm at thawing; however, significant differences in motility (P < 0.001, n = 10 fish semen) were observed at further post-activation times, the motility decrease being faster in thawed sperm. At the experimental scale, triplicate inseminations of 2-mL aliquots (approximately 2 000 eggs) showed a significant fertility decay of thawed sperm compared to that of fresh sperm (P < 0.01, n = 12 fish semen) when a discriminating 35.10(3) spermatozoa to egg ratio was applied.... |
| Tipo: Text |
Palavras-chave: Dicentrarchus labrax; Sea bass; Cryobank; Insemination; Spermatozoa; Cryopreservation; Dicentrarchus labrax; Loup; Banque de sperme; Insémination; Cryoconservation; Cryoconservation. |
| Ano: 1998 |
URL: http://archimer.ifremer.fr/doc/1998/publication-866.pdf |
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| Peruzzi, Stefano; Chatain, Beatrice; Menu, Bruno. |
| The nuclear DNA content in Dicentrarchus labrax, Sparus aurata, Liza ramada, and Anguilla anguilla, was measured by flow cytometric analysis. Male human leukocytes were used as internal reference cells. Nucleated fish erythrocytes were stained simultaneously to human leukocytes with a staining buffer containing propidium iodide (PI). Nuclear DNA contents of target species were estimated in relation to an assigned value of 7.0 pg DNA for male human leukocytes. The DNA content/nucleus (+/- CI) was 1.55 +/- 0.02 pg for D. labrax, 1.90 +/- 0.03 pg for S. aurata, 1.57 +/- 0.02 pg for L. ramada, and 2.43 +/- 0.04 pg for A. anguilla. Intraspecific DNA content variations ranged from 0-9% and averaged around 4% in all taxa. The results did not evidence significant... |
| Tipo: Text |
Palavras-chave: Anguilla anguilla; Liza ramada; Sparus aurata; Dicentrarchus labrax; Teleosts; DNA content. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-411.pdf |
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| Peruzzi, Stefano; Varsamos, Stamatis; Chatain, Beatrice; Fauvel, Christian; Menu, Bruno; Falguiere, Jean-claude; Severe, Armelle; Flik, Gert. |
| The purpose of this study was to determine whether diploid and triploid sea bass differed in terms of main haematological and physiological characteristics. Diploid and triploid fish were produced by sub-optimal pressure treatments and held in communal environments under standard rearing conditions. Total red blood cell count (RBCC), haemoglobin concentration (Hb), hematocrit (Hct), mean cell volume (MCV), mean cellular haemoglobin content (MCH), mean cell haemoglobin concentration (MCHC), plasma metabolites, osmotic pressure, gill Na+/K+-ATPase activity, electrolytes, cortisol, and 3,5,3'-triiodo-L-thyronine (T-3) were measured and compared. Triploidisation in sea bass led to an increase in erythrocyte size (32% in cytoplasm surface area, and 50% in... |
| Tipo: Text |
Palavras-chave: Dicentrarchus labrax; Sea bass; Triploid; Diploid; Physiology; Haematology. |
| Ano: 2005 |
URL: https://archimer.ifremer.fr/doc/2005/publication-508.pdf |
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| Menu, Bruno; Girin, Michel. |
| A batch of red mullet spawners, held in captivity, spawned for 3 years in a 20 m3 tank. The eggs were incubated between 9 and 18° C, and hatched normally. The relationship between incubation time and temperature was calculated. Several rearing trials were performed at 19° C, in 150 l tanks. SurvivaIs up to 32 days were obtained, but metamorphosis was not yet completed at that age. The corresponding growth curves were drawn, and the Iarval development of the species was studied in detail. |
| Tipo: Text |
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| Ano: 1978 |
URL: http://archimer.ifremer.fr/doc/00138/24885/22976.pdf |
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